Bacillus subtilis 3108株の産生する生澱粉分解性α-アミラーゼの特性(物質生命化学科)

Abstract

α-Amylases from Bacillus sp. strains digesting raw starch were observed to adsorb to raw starch and α-cyclodextrin (CD) Sepharose. Raw starch digesting and saccharyfying α-amylases was purified from Bacillus subtilis IFO 3108 (RSA-1, MM 67 kDa). BSA1 was able to adsorb to α-CD Sepharose and to digest raw starch. The other amylase from the strain was unable to adsorb to α-CD Sepharose CL-6B and failed to digest raw starch. The digest of raw corn starch was specifically inhibited by α-CD for BSA1 (Ki, 0.44mM). The adsorption ability of BSA1 to α-CD Sepharose had not lost upon protease treatment. Results indicate that although raw starch affinity domain (SAD) involved is present in BSA1, the structure and mode of binding of SAD are different from other amylases.