海苔からのポルフィランの抽出(物質生命化学科)

Abstract

Compressed hot-water (CHW) extraction method was investigated to obtain a high purity of Porphyran (POR) from lave. The CHW extraction condition was for 15 minutes at 121℃ Following the CHW extraction, three procedure were tried to isolate a solid extract and refine the POR from laver. The first was as follows; the solution of extracts obtained by CHW extraction was dried without father purification. The yield of solid extracts was about 45% base on law laver. Regrettably, the solid extracts was stained brown. The second was as follows; the powder of active carbon was added to the solution of CHW extracts to remove colored impurities. However, the colored impurities were still remained and a portion of activated carbon might be left in the products. The third was as follows; to prevent admixture of colored impurities in the product, pretreatment using acetone to remove lipophilic component against laver was applied before CHW extraction. After CHW extraction, aqueous sodium acetate solution was added to change pH of the obtained aqueous solution to alkaline side and ethyl alcohol was subsequently added to precipitate pure POR. The product obtained by third method was white powder and the yield of product based on law laver was 43%.